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Behavioral Objectives - Zeta
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Explain the molecular basis of Mendelian genetics. Describe and identify
examples of the relationship between DNA, protein and heredity traits.
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Explain how mutations affect heredity through the DNA, protein, heredity
trait relationship.
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Relate DNA to genes and chromosomes.
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Describe the structural features of the nucleic acid molecules DNA
and RNA using the following terms: double helix, polymer, nucleotides,
sugars, phosphate and nitrogen-containing bases, complementary base pairs,
complementary strands.
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Describe the structural features of protein molecules which are responsible
for forming a specific enzyme (primary, secondary, and tertiary structure,
role of amino acids, differences between amino acids (R group)).
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Contrast between the nucleic acids DNA and RNA.
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Describe DNA replication. Given the sequence of bases in a piece of DNA
draw its complementary strand.
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Identify several factors involved in DNA replication - energy source; free
nucleotides; enzymes such as helicase, DNA polymerase, and ligases;
replication fork; semiconservative replication.
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Explain the direction of synthesis of the complementary DNA strand.
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List the differences (structural and function) between ribosomal RNA, messenger
RNA, and transfer RNA.
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Explain the role of the 3 RNA's (tRNA, rRNA, and mRNA) during protein synthesis.
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Contrast between codons and anticodons.
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Explain and show transcription and translation.
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Given the sequence of bases on a piece of DNA and the genetic code (codons
of mRNA) for the amino acids, determine the mRNA which will be formed and
the sequence (primary structure) of amino acids in the protein formed.
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Identify the start and stop codons.
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Define Biotechnology. Describe DNA recombination as it may occur
in nature.
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Describe the following parts of a bacteria cell: cytoplasm, circular
chromosome, plasmid.
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Describe transformation.
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Explain how a DNA library is constructed.
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Explain the origin and purpose of restriction enzymes and the mechanics
of cleaving DNA.
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Define sticky ends; explain their part in recombination.
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Descibe the function of DNA ligase in recombination and in
nature.
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Catalog the properties of engineered plasmids (ampicillin resistant
gene).
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Describe how DNA probes are used to locate bacteria containing
plasmids with desired genes.
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Explain the process of cloning (amplification).
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Describe the polymerase chain reaction in making copies of specific stretches
of DNA.
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List some of the uses of amplified genes.
Describe RFLPs and explain how they are used to locates genes. List
other uses of RFLPs.
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List examples of genetic engineering in relation to agriculture and medical
uses in humans. .
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Last modified October 29, 1997. |